The article is the students conducting a lab that reflects the foundations of DNA fingerprinting. Parameters for the individual and combined primers were initially figured out. The students then used DNA samples from soda cans, bottles, cheek swabs, and plastic spoons. The resulting polymerase chain reaction products were analyzed by gel elctrophoresis. The student could subsequently determine and analyze the DNA fingerprint.
A collection swab was used to obtain the DNA from the different samples. The samples were centrifuged many times at different temperatures. The top DNA layer was taken out and placed into another tube. It was centrifuged again and the supernatant was taken out. The tubes were stored at cold temperatures. PCR analysis was conducted in the laminar flow hood. In the analysis, there was a positive control, a negative control, and the actual DNA sample. Using the combined primers, the DNA samples were increased by many cycles with the soda can and bottle samples. PCR product or marker were loaded into the gel and elcetrophoresed. A picture was taken using the gel photodocumentation system. for the comparison and analysis of the gel.
Each individual primer was intially tested using a control human DNA from monocytic cells. The DNA from the various samples were tested against the primer combination. The soda can, bottle and and spoon all had one PCR product two primers and two product for another primers. These products were in the expected ranges of the loci tested. The PCR products for the three loci were the same size for the spoon, soda can, and bottle, which validataed that the DNA was from one person.
Akash Sha
Carlson K. DNA Fingerprinting in a Forensic Teaching Experiment. American Biology Teacher. August 2008:29-33.
The article is the students conducting a lab that reflects the foundations of DNA fingerprinting. Parameters for the individual and combined primers were initially figured out. The students then used DNA samples from soda cans, bottles, cheek swabs, and plastic spoons. The resulting polymerase chain reaction products were analyzed by gel elctrophoresis. The student could subsequently determine and analyze the DNA fingerprint.
A collection swab was used to obtain the DNA from the different samples. The samples were centrifuged many times at different temperatures. The top DNA layer was taken out and placed into another tube. It was centrifuged again and the supernatant was taken out. The tubes were stored at cold temperatures. PCR analysis was conducted in the laminar flow hood. In the analysis, there was a positive control, a negative control, and the actual DNA sample. Using the combined primers, the DNA samples were increased by many cycles with the soda can and bottle samples. PCR product or marker were loaded into the gel and elcetrophoresed. A picture was taken using the gel photodocumentation system. for the comparison and analysis of the gel.
Each individual primer was intially tested using a control human DNA from monocytic cells. The DNA from the various samples were tested against the primer combination. The soda can, bottle and and spoon all had one PCR product two primers and two product for another primers. These products were in the expected ranges of the loci tested. The PCR products for the three loci were the same size for the spoon, soda can, and bottle, which validataed that the DNA was from one person.
Akash Sha
Carlson K. DNA Fingerprinting in a Forensic Teaching Experiment. American Biology Teacher. August 2008:29-33.